Rat mesenchymal stem cells were isolated from bone marrow collected from an older adult (16 month) male Sprague Dawley rat. Cells were cultured in Cell Expansion Media (Minimum Essential Medium (MEM), Alpha 1X (Corning Cellgro; Catalog # 15-012-CV) with 1% MEM Nonessential Amino Acids (Corning Cellgro; Catalog # 25-025-Cl), 1% Corning glutagro Supplement (Corning; Catalog # 25-015-Cl), 1% Antibiotic-Antimycotic Solution (Anti-Anti (100X); gibco; Catalog # 15240-062), and 10% Hyclone Fetal Bovine Serum (Canada characterized FBS; (HyClone; Catalog # SH30396.03)). Cells were cultured in a cell culture incubator at 37oC with 5% CO2, and were passaged (Passage 3 (P3)) for purity and cryopreserved. P3 cells can be expanded for a minimum of 5 passages.
Suggested Cell Culture Medium
JangoCell suggests a modified version of Minimum Essential Medium (MEM), Alpha 1X. Details of the media are specified in the Product Data Sheet and will be provided with shipment.