Adipose-derived stem cells were isolated from an older adult (16 month) male Sprague Dawley rat. Cells were cultured in Cell Expansion Media (Minimum Essential Medium (MEM), Alpha 1X (Corning Cellgro; Catalog # 15-012-CV) with 1% MEM Nonessential Amino Acids (Corning Cellgro; Catalog # 25-025-Cl), 1% Corning glutagro Supplement (Corning; Catalog # 25-015-Cl), 1% Antibiotic-Antimycotic Solution (Anti-Anti (100X); gibco; Catalog # 15240-062), and 10% Hyclone Fetal Bovine Serum (Canada characterized FBS; (HyClone; Catalog # SH30396.03)). Cells were cultured in a cell culture incubator at 37oC with 5% CO2, and were passaged (Passage 2 (P2)) for purity and cryopreserved. P2 cells can be expanded for a minimum of 5 passages.
Suggested Cell Culture Medium
JangoCell suggests a modified version of Minimum Essential Medium (MEM), Alpha 1X. Details of the media will be provided with shipment.